Emergence of fosfomycin resistance among isolates of multidrug-resistant Enterobacter species at a tertiary care hospital in Dhaka, Bangladesh

Background: The emergence of multidrug-resistant Enterobacter species as a worrying resistant pathogen seriously threatened human health. rising rate resistance to commonly used antibiotics limit the choice hence it is urgent evaluate antimicrobial activity older drug like Fosfomycin. Objective: study aimed seek frequency fosfomycin in clinical isolates and detect gene along with antibiotic pattern. Methods: This cross-sectional was conducted Department Microbiology tertiary care hospital Bangladesh from July 2018 June 2019. spp. isolated total 350 samples by standard microbiological method. Antibiotic susceptibility performed disk diffusion technique. minimum inhibitory concentration (MIC) were determined agar dilution Fosfomycin fosA, fosA3, fosA4, fosA5, fosB, fosB2, fosC, fosC2 fosX among fosfomycin‑resistant spp detected polymerase chain reaction (PCR) using specific primer. Sequencing fosA fosA₅ capillary method, nucleotide sequence has been deposited GenBank. Results: Out 28 7 (25%) fosfomycin-resistant strains, 4 (57.14%) urine samples. Fifteen (53.57%) disc All MDR. A significant rise MIC found between 256µg/ml- ≥4096 µg/ml fosfomycin. PCR revealed that 100% are positive for 71.43% 28.57% fosB₂ respectively. established FosA family glutathione transferase gene. Conclusion: results this showed high proportion irrespective usage Bangladesh. emerging Medical Res Counc Bull 2022; 48(3): 203-210